期刊
ANALYTICAL CHEMISTRY
卷 75, 期 7, 页码 1671-1675出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac020579e
关键词
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资金
- NIGMS NIH HHS [T32 GM145304] Funding Source: Medline
This paper describes a self-contained integrated microfluidic system that can separate motile sperm from small samples that are difficult to handle using conventional sperm-sorting techniques. The device isolates motile sperm from nonmotile sperm and other cellular debris, based on the ability of motile sperm to cross streamlines in a laminar fluid stream. The device is small, simple, and disposable yet is an integrated system complete with sample inlets, outlets, sorting channel, and a novel passively driven pumping system that provides a steady flow of liquid; it requires no external power source or controls. The device fulfills a need in clinical settings where small amounts of sperm need to be sorted. It also opens the way for convenient bioassays based on sperm motility including at-home motile sperm tests.
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