期刊
AMERICAN JOURNAL OF HUMAN GENETICS
卷 72, 期 4, 页码 931-939出版社
CELL PRESS
DOI: 10.1086/374176
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资金
- NHGRI NIH HHS [U01 HG02138-04] Funding Source: Medline
- NINDS NIH HHS [R01 NS43264-01, R01 NS043264] Funding Source: Medline
Mutations in the dystrophin gene result in both Duchenne and Becker muscular dystrophy (DMD and BMD), as well as X-linked dilated cardiomyopathy. Mutational analysis is complicated by the large size of the gene, which consists of 79 exons and 8 promoters spread over 2.2 million base pairs of genomic DNA. Deletions of one or more exons account for 55%-65% of cases of DMD and BMD, and a multiplex polymerase chain reaction method-currently the most widely available method of mutational analysis-detects similar to98% of deletions. Detection of point mutations and small subexonic rearrangements has remained challenging. We report the development of a method that allows direct sequence analysis of the dystrophin gene in a rapid, accurate, and economical fashion. This same method, termed SCAIP (single condition amplification/internal primer) sequencing, is applicable to other genes and should allow the development of widely available assays for any number of large, multiexon genes.
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