期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 275, 期 1-2, 页码 113-121出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0022-1759(03)00009-7
关键词
flow cytometry; cell cycle analysis; DNA staining; immunofluorescence
资金
- NCI NIH HHS [CA08748] Funding Source: Medline
- NIAID NIH HHS [AI33940] Funding Source: Medline
In this manuscript, we describe modifications to a commercial three-laser benchtop flow cytometer, as well as relevant biological methods, that allow analysis of up to five immunofluorescent parameters together with an ultraviolet (UV)-excitable DNA stain. This method allows expanded capacity for multiparameter immunophenotyping of complex mixed cell populations, together with accurate measurements of DNA content (cell cycle) or cell viability, on a stable, end-user operated platform. (C) 2003 Elsevier Science B.V. All rights reserved.
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