期刊
ACS APPLIED MATERIALS & INTERFACES
卷 7, 期 37, 页码 20568-20575出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b04290
关键词
gold nanoparticle; size; protein corona; cell line; uptake level; endocytic pathways
资金
- National Basic Research Program of China (973 Program) [2014CB931900]
- National Natural Science Foundation of China [21207164, 81573178, 31400862]
- Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions
- Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
The interaction at nanobio is a critical issue in designing safe nanomaterials for biomedical applications. Recent studies have reported that it is nanoparticle-protein corona rather than bare nanoparticle that determines the nanoparticle cell interactions, including endocytic pathway and biological responses. Here, we demonstrate the effects of protein corona on cellular uptake of different sized gold nanoparticles in different cell lines. The experimental results show that protein corona significantly decreases the internalization of Au NPs in a particle size- and cell type-dependent manner. Protein corona exhibits much more significant inhibition on the uptake of large-sized Au NPs by phagocytic cell than that of small-sized Au NPs by nonphagocytic cell. The endocytosis experiment indicates that different endocytic pathways might be responsible for the differential roles of protein corona in the interaction of different sized Au NPs with different cell lines. Our findings can provide useful information for rational design of nanomaterials in biomedical application.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据