期刊
ANALYTICAL BIOCHEMISTRY
卷 315, 期 1, 页码 57-66出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S0003-2697(02)00702-9
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资金
- NCRR NIH HHS [RR-08119, P41 RR008119, P41 RR008119-12, P41 RR008119-13, P41 RR008119-14] Funding Source: Medline
- NIBIB NIH HHS [R01 EB000682-05, EB000682, R01 EB000682, R01 EB000682-03, R01 EB000682-04] Funding Source: Medline
We examined the fluorescence spectral properties of Cy3- and Cy5-labeled oligonucleotides at various distances from the surface of silver island films. The distance to the surface was controlled by alternating layers of biotinylated bovine serum albumin (BSA) and avidin, followed by binding of a biotinylated oligonucleotide. The maximum enhancement of fluorescence near a factor of 12 was observed for the first BSA-avidin layer, with the enhancement decreasing to 2-fold for six layers. The minimum lifetimes were observed for the first BSA-avidin layer, and were about 25-fold shorter than on quartz slides without silver, with the lifetimes being about 2-fold shorter for six BSA-avidin layers. These results suggest that maximum fluorescence enhancements occur about 90 Angstrom from the silver surface, a distance readily obtained by one or two layers of proteins. (C) 2003 Elsevier Science (USA). All rights reserved.
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