期刊
JOURNAL OF STRUCTURAL BIOLOGY
卷 142, 期 1, 页码 218-231出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S1047-8477(03)00052-2
关键词
protein crystallization; counterdiffusion; cryocrystallography; high throughput; crystallization cassette
Counterdiffusion crystallization in capillary is a very simple, cost-effective, and practical procedure for obtaining protein crystals suitable for X-ray data analysis. Its principles have been derived using well-known concepts coupling the ideas of precipitation and diffusion mass transport in a restricted geometry. The counterdiffusion process has been used to simultaneously screen for optimal conditions for protein crystal growth, incorporate strong anomalous scattering atoms, and mix in cryogenic solutions in a single capillary tube. The crystals obtained in the capillary have been used in situ for X-ray analysis. The implementation of this technique linked to the advancement of current crystallography software leads to a powerful structure determination method consolidating crystal growth, X-ray data collection, and ab initio phase determination into one without crystal manipulation. We review the historical progress of counterdiffusion crystallization, its application to X-ray crystallography, and ongoing too] development for high-throughput protein structure determination. (C) 2003 Elsevier Science (USA). All rights reserved.
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