期刊
ARTIFICIAL ORGANS
卷 34, 期 12, 页码 1150-1153出版社
WILEY
DOI: 10.1111/j.1525-1594.2009.00988.x
关键词
BMP-2 delivery; Bone formation; Fibrin gel; Heparin-conjugated poly(lactic-co-glycolic acid) nanosphere
资金
- Stem Cell Research Center [SC 3220]
- Korea Science and Engineering Foundation, the Ministry of Education, Science and Technology, Republic of Korea [2009-0080769, 2009-0092213]
- National Research Foundation of Korea [2009-0080769, 2009-0092213] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Bone morphogenetic protein-2 (BMP-2) induces bone regeneration in a dose-dependent manner, with higher doses of BMP-2 inducing greater bone formation. Previously, we showed that long-term delivery of BMP-2 provides better ectopic bone formation than short-term delivery of an equivalent dose. In the present study, we investigated the efficacy of orthotopic bone formation over a range of BMP-2 doses, using different delivery modes. Heparin-conjugated poly(lactic-co-glycolic acid) nanospheres suspended in fibrin gel were used as a long-term delivery system, and fibrin gel was used as a short-term delivery system. Different doses of BMP-2 were delivered to mouse calvarial defects using either long-term or short-term delivery systems. Eight weeks after treatment, bone regeneration was evaluated by histomorphometry. For both delivery systems, bone regeneration increased as the BMP-2 dose increased up to 1 mu g and did not increase beyond this dose. Importantly, at BMP-2 doses higher than 1 mu g, long-term delivery resulted in much greater bone formation than short-term delivery. This study shows that long-term delivery of BMP-2 is more effective at enhancing orthotopic bone formation than short-term delivery over a range of doses.
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