Multiplex PCR for detection of aminoglycoside resistance genes in enterococci

A multiplex PCR procedure for detecting the aminoglycoside resistance genes aac(6')-Ie-aph(2)-Ia, aph(2)-Ib, aph(2)-Ic, aph(2)-Id, aph(3')-IIIa, and ant(4')-Ia was evaluated and found to determine accurately the presence of these genes in enterococci.