期刊
EXPERIMENTAL DERMATOLOGY
卷 12, 期 2, 页码 204-212出版社
WILEY
DOI: 10.1034/j.1600-0625.2003.00044.x
关键词
CD44; melanoma; adhesion; regression; tumor-infiltrating lymphocytes
类别
CD44 is a family of cell-surface receptors on human lymphocytes that act as co-stimulatory molecules leading to the induction of effector functions in T cells. We have analyzed primary cutaneous malignant melanomas with clinical and histologic signs of tumor regression using immunohistochemistry and observed the predominant expression of the CD44 variant isoform v10 on CD3 CD4/CD8 co-expressing tumor-infiltrating lymphocytes (TIL). We further analyzed the role of CD44v10 in adhesion of lymphocytes to human melanoma cells. In contrast to CD44(-) lymphatic cells, CD44v10(+) lymphatic cells strongly bound to cultured human melanoma cells and to frozen tissue samples of melanomas. Antibody blocking studies revealed a hyaluronan, integrin-, and selectin-independent pathway of adhesion. Furthermore, CD44v10(+) lymphatic cells exhibited significantly higher invasiveness in three-dimensional collagen matrices as compared with CD44H(+) and CD44-negative lymphocytes. These results indicate that expression of CD44v10 on TIL may mediate adhesion to melanoma cells and result in gain of novel invasive properties.
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