期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 53, 期 1, 页码 131-135出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0167-7012(02)00232-4
关键词
fluorescence spectroscopy; Nile red; medium-chain-length; polyhydroxyalkanoate; short-chain-length
An approach for rapid differentiation between short-chain-length (scl) and medium-chain-length (mcl) polyhydroxyalkanoate (PHA) producers was developed. Polyhydroxyalkanoate-accumulated bacterial cells stained with Nile red were suspended in water and subjected to fluorescence spectroscopy at a fixed excitation wavelength of 488 nm. The scl-PHA-accumulated bacteria revealed a maximum emission wavelength at 590 nm, and for mcl-PHA producers were seen at a wavelength of 575 nm. Combining Nile red staining and fluorescence spectroscopy, the accumulated PHA granules could be rapidly differentiated into scl-PHA and mcl-PHA from the intact cells. (C) 2002 Elsevier Science B.V. All rights reserved.
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