期刊
BIOSENSORS & BIOELECTRONICS
卷 18, 期 4, 页码 473-481出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/S0956-5663(02)00146-X
关键词
immunosensor; carbon-paraffin electrode; adsorption immobilization; complement III
An electrochemical immunosensor based on the adsorption of anti-complement III antibody onto an electrochemical pretreated carbon-paraffin electrode has been proposed for the detection of complement III (C-3). The competitive immunoassay format was adopted with horseradish peroxide-C-3 (HRP-C-3) as a tracer, 3,3'5,5'-tetramethylbenzidine (TMB) and hydrogen peroxide as the enzyme substrates. In order to measure the amount of HRP-C3 binding onto the electrode surface, the product of the enzyme catalytic reaction was detected at 100 mV (vs. Ag/AgCl reference electrode). The system was optimized to realize a reliable determination Of C-3 in the range of 0.06-10 mug/ml. It exhibits some advantages, such as simplicity of fabrication, rapidity of measurement, and satisfactory sensitivity and reproducibility. (C) 2003 Elsevier Science B.V. All rights reserved.
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