Micro-porous membrane liquid-liquid extraction as an enrichment step prior to nonaqueous capillary electrophoresis determination of sulfonylurea herbicides

A method based on micro-porous membrane liquid-liquid extraction (MMLLE) enrichment and nonaqueous capillary electrophoresis (CE) separation, was established for the analysis of sulfonylurea herbicides in water samples. After MMLLE, the analyte trapped in the chloroform was treated mildly with nitrogen flow to dryness and then dissolved in 200 mul of 4 mM Tris methanol solution for CE analysis. Five sulfonylurea herbicides were separated by nonaqueous CE with Tris/acetate of methanol solution as the run buffer. MMLLE related parameters such as organic solvent used as acceptor, sample flow rate, sample pH, enrichment time, and salt effect were investigated with tribenuron methyl (TBM) as a model compound. Results showed that with a sample flow rate of 3.0 ml min(-1) and an enrichment time of 20 min, the proposed method has good linear relationship over the scope of 1-15 ng ml(-1) with related coefficient of R-2 = 0.9911, and a detection limit of 0.4 ng ml(-1). This method was applied to determine TBM in realworld water samples with recoveries over the range of 89-97%. (C) 2002 Elsevier Science B.V. All rights reserved.