Elevated serum levels of thioredoxin in patients with acute exacerbation of asthma

The pathogenesis of bronchial asthma is chronic airway inflammation caused by immune cells such as T lymphocytes and eosinophils. Eosinophils release cytotoxic products including reactive oxygen species at the site of inflammation, leading to epithelia] damage. Human thioredoxin (TRX), a redox-regulating protein with antioxidant activity, is induced and secreted from cells by oxidative stress. This study was undertaken to investigate the clinical significance of TRX in the pathogenesis of asthma. We collected blood samples from 48 patients with bronchial asthma with or without attack, and measured serum ECP and pulmonary function as well as serum TRX. The serum TRX levels in patients with asthma were significantly increased in patients with mild (34.63 [28.40-42.73] ng/ml, medians with 25 and 75%) interquartiles, P = 0.0064) and moderate (38.83 [35.14-50.80] ng/ml, P = 0.0017) asthma attacks compared with those during the asymptomatic period. The serum TRX levels were inversely correlated with FEV1.0% (r = -0.44, P = 0.039) and %PEF (r = -0.49, P = 0.020) during attack. There was a significant correlation between the serum TRX and the serum eosinophil cationic protein (rs = 0.32, P = 0.016). These findings suggest that serum TRX is related to the state of asthma exacerbation and allergic inflammation. (C) 2003 Elsevier Science B.V. All rights reserved.