期刊
FEBS LETTERS
卷 540, 期 1-3, 页码 181-187出版社
WILEY
DOI: 10.1016/S0014-5793(03)00261-8
关键词
apolipoprotein E receptor 2; EA.hy926 cells; nitric oxide synthase; PI3-kinase; tyrosine phosphorylation
Although apolipoprotein E3 (apoE3) is atheroprotective, two common isoforms, apoE2 and apoE4, produce recessive and dominant hyperlipidaemias, respectively. Using a fluorescent assay, we report herein that apoE3 particles secreted from recombinant cells stimulate more nitric oxide release in cultured human EA.hy926 endothelial cells than apoE2 or apoE4 (141% more than controls vs. 61 or 11%). Phosphatidylinositol (PI) 3-kinase inhibitors suppressed the apoE effect, while apoE receptor 2 (apoER2) was tyrosine phosphorylated. We conclude that apoE stimulates endothelial nitric oxide release in an isoform-dependent manner, and propose that tyrosine phosphorylation of apoER2 initiates PI3-kinase signalling and activation of nitric oxide synthase. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
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