期刊
AQUACULTURE
卷 220, 期 1-4, 页码 203-218出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0044-8486(02)00353-8
关键词
linkage map; microsatellite; AFLP; Paralichthys olivaceus
We report the first genetic linkage map of the Japanese flounder (Paralichthys olivaceus) constructed with 111 microsatellite markers and 352 AFLP fragments. The parental male linkage map consisted of 25 linkage groups while the female map consisted of 27 groups, with an average resolution of 8 and 6.6 cM, respectively. We have identified linkage among 96% of the markers and the total map length was estimated to be around 1000-1200 W. This study reports the first low-density linkage map for the Japanese flounder and describes differences in sex recombination. Recombination rates were higher in male flounder compared to the female (7.4:1), a rare condition among vertebrates. This map is a starting point for the mapping of single loci and quantitative traits in flatfish species. (C) 2003 Elsevier Science B.V All rights reserved.
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