期刊
JOURNAL OF CHROMATOGRAPHY A
卷 993, 期 1-2, 页码 79-87出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(02)01817-4
关键词
plant material; Brassica napus; Arabidopsis thaliana; aminocyclopropanecarboxylic acid; carboxylic acids
We developed a new method for the determination of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) using quantitative GC-negative chemical ionisation MS as a detection and quantification system, in combination with isotope dilution using [H-2(4)]ACC and an off-line solid-phase extraction. By derivatisation with pentafluorobenzyl bromide, ACC could easily be detected with m/z 280 being the most abundant ion. Determination of this component resulted in a detection limit of 10 fmol and a linear fit in the 100 fmol-100 pmol range. The combination of a rapid, high yield purification method with a stable derivatisation procedure and a sensitive detection method allowed the detection of ACC in samples as low as 100 mg fresh mass. (C) 2002 Elsevier Science B.V. All rights reserved.
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