期刊
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
卷 39, 期 3, 页码 185-190出版社
INFORMA HEALTHCARE
DOI: 10.3109/10731199.2010.533124
关键词
HRP; dextran aldehyde; modification; Coomassie Brilliant Blue R-250; decolorization
Horseradish peroxidase (EC 1.11.1.7) was chemically modified by periodate-activated dextran. The activities of free and modified enzyme against organic-aqueous interface and some chemicals were determined. Modified HRP remained fully active in the presence of organic solvent for 4 h. However, the unmodified enzyme lost 50% of its activity within the first 2 h. The effects of possible inhibitors on enzyme activity were investigated. In addition, Coomassie Brilliant Blue R-250 was efficiently decolorized using the free and modified HRP. After 5 minutes of treatment, the color removal of dye was 80-90%. Modified HRP showed effective performance compared to free HRP.
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