期刊
VIROLOGY
卷 309, 期 1, 页码 18-31出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S0042-6822(03)00009-6
关键词
PRRS virus; interferon-gamma; swine T cell; polarization of immunity; neutralizing antibodies; adjuvant; cellular immunity; ELISPOT
类别
Infection of swine with virulent porcine reproductive and respiratory syndrome (PRRS) virus induced a rapid, robust antibody response that comprised predominantly nonneutralizing antibodies and waned after approximately 3 months. In contrast, the initial onset of virus-specific interferon (IFN)-gamma-secreting cells (SC) in the pig lymphocyte population remained at a fairly low level during this period and then increased gradually in frequency, plateauing at 6 months postinfection. A similar polarization of the host humoral and cellular immune responses was also observed in pigs immunized with a PRRS-modified live virus (MLV) vaccine. Even coadministration of an adjuvant that enhanced the immune response to a pseudorabies (PR) MLV vaccine failed to alter the induction of PRRS virus-specific IFN-gamma SC (comprising predominately CD4/CD8alpha double positive memory T cells with a minority being typical CD4(-)/CD8alphabeta(+) T cells) and the generation of neutralizing antibodies. Moreover, unlike inactivated PR virus, nonviable PRRS virus did not elicit virus-neutralizing antibody production. Presumably, an intrinsic property of this pathogen delays the development of the host IFN-gamma response and preferentially stimulates the synthesis of antibodies incapable of neutralization. (C) 2003 Elsevier Science (USA). All rights reserved.
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