期刊
JOURNAL OF CHROMATOGRAPHY A
卷 994, 期 1-2, 页码 85-92出版社
ELSEVIER
DOI: 10.1016/S0021-9673(03)00486-2
关键词
monolithic columns; chlorophylls; chlorophyllides; pheophytins; pheophorbides
A monolithic C-18-bonded silica rod column (Merck Chromolith) was applied to the separation of mixtures of chlorophylls a and b and their derivatives originated by hydrolysis of the phytyl ester linkage (chlorophyllides), loss of the central Mg atom (pheophytins), or both processes (pheophorbides). Mobile phases containing two different ion-pair reagents, ammonium acetate and buffered pyridine, were tested. Both eluents achieved the resolution of the eight pigments in less than 5 min. The method based on the pyridine-containing mobile phase was applied to the separation of chlorophylls and their green coloured degradation products in senescing leaves of deciduous trees, green fruits and a marine microalga. (C) 2003 Elsevier Science B.V. All rights reserved.
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