期刊
JOURNAL OF CRANIOFACIAL SURGERY
卷 14, 期 3, 页码 348-355出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/00001665-200305000-00013
关键词
calvarial development; calvarial morphogenesis; osteoblast biology; mechanotransduction; constant equibiaxial strain
类别
资金
- NIDCR NIH HHS [R01 DE 14526, R01 DE 13028, R01 DE 13194] Funding Source: Medline
Mechanical tensile strain is believed to play an important role in regulating calvarial morphogenesis. To better understand the effects of mechanical strain on pathologic calvarial growth, we applied 10% constant equibiaxial tensile strain to neonatal rat calvarial osteoblast cultures and examined cellular proliferation, cytokine production, and extracellular matrix molecule expression. Mechanical strain markedly increased osteoblast proliferation as demonstrated by increased proliferating cell nuclear antigen (PCNA) protein. In addition, both transforming growth factor-beta1 (TGF-beta1) mRNA expression and fibroblast growth factor-2 (FGF-2) protein production were increased with exposure to strain. Moreover, mechanical strain induced expression of the extracellular matrix molecule collagen IalphaI. To further explore the relationship between mechanotransduction, osteogenesis, and angiogenesis, we examined the effect of mechanical strain on calvarial osteoblast expression of vascular endothelial growth factor (VEGF). Interestingly, we found that mechanical strain induced a rapid (within 3 hrs) increase,in osteoblast VEGF expression. These data suggest that constant equibiaxial tensile strain-induced mechanotransduction can influence osteoblasts to assume an osteogenic and angiogenic phenotype, and these findings may have important implications for understanding the mechanisms of pathologic strain-induced calvarial growth.
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