期刊
ELECTROANALYSIS
卷 15, 期 7, 页码 629-634出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.200390079
关键词
electrochemical oxidation of DNA; electrochemical impedance; guanine; gold
Electrochemical CV and SWV studies were performed with double stranded DNA from salmon testes (dsDNA) and single stranded DNAs, containing 25 nucleotides (ssDNA) directly adsorbed at polycrystalline An electrodes. A distinct oxidation peak at + 730 mV (SWV, scan rate 0.248 V s(-1)) or at + 730 - + 780 mV (CV, scan rate from 0.3 to 1 V s(-1)) was obtained with DNA-modified Au electrodes after a time-dependent prepolarization step at a positive potential value, i.e., at + 500 mV (vs. Ag \ AgCl), performed with the DNA-modified Au electrodes dipped in a blank buffer solution. No electrochemical activity was detected when ssDNA, containing no guanines, was used for adsorptive modification of the Au electrodes. Electrochemical impedance measurements registered a possible reorganization of the adsorbed DNA layer in the course of the prepolarization, accompanied by decreasing in-phase impedance. ne results enable us to relate the oxidation process observed at the DNA-modified Au electrodes with the oxidation of guanine residues in DNA.
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