期刊
EMBO JOURNAL
卷 22, 期 9, 页码 2127-2134出版社
OXFORD UNIV PRESS
DOI: 10.1093/emboj/cdg212
关键词
circadian clock; cyanobacteria; KaiB; KaiC phosphorylation; localization
资金
- NCIPC CDC HHS [U17 CE002005] Funding Source: Medline
In the cyanobacterium Synechococcus elongatus PCC 7942, the KaiA, KaiB and KaiC proteins are essential for generation of circadian rhythms. We quantitatively analyzed the intracellular dynamics of these proteins and found a circadian rhythm in the membrane/cytosolic localization of KaiB, such that KaiB interacts with a KaiA-KaiC complex during the late subjective night. KaiB-KaiC binding is accompanied by a dramatic reduction in KaiC phosphorylation and followed by dissociation of the clock protein complex(es). KaiB attenuated KaiA-enhanced phosphorylation both in vitro and in vivo. Based on these results, we propose a novel role for KaiB in a regulatory link among subcellular localization, protein-protein interactions and post-translational modification of Kai proteins in the cyanobacterial clock system.
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