期刊
NEUROSCIENCE RESEARCH
卷 46, 期 1, 页码 105-117出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/S0168-0102(03)00038-5
关键词
squid; voltage-dependent calcium channel; beta subunits; functional expression; tissue distribution; phosphorylation; protein kinase C; protein kinase CK2
cDNAs that encode beta subunits of voltage-dependent Ca2+ channel were cloned from the optic lobe of the squid Loligo bleekeri. The subunits, LoCa(v)beta(1a) and LoCa(v)beta(1a) are 96% identical in amino acid sequence. The sole sequence differences are in the N-terminal region and in a five amino acid insertion in the central region of LoCa(v)beta(1a). RT-PCR revealed that LoCa(v)beta(1a) and LoCa(v)beta(1a) transcripts were expressed mainly in the optic lobe and stellate ganglion, and more weakly in mantle muscle, systemic heart, gill, branchial heart, stomach and liver. Coexpression of LoCa(v)beta(1a) or LoCa(v)beta(1a) with mammalian Ca(v)2.3 and alpha(2)/delta subunits in the Xenopus oocyte resulted in high-voltage-activated currents, and showed slow current inactivation and moderate steady-state inactivation. Comparison of the squid subunits with four mammalian beta subunits, beta(1b), beta(2a), beta(3) and beta(4), demonstrated that the modulatory effects of the beta subunits on steady-state inactivation kinetics were beta(3) < beta(4) approximate to beta(1b) < LoCa(v)beta(1a) approximate to LoCa(v)beta(1a) < beta(2a). LoCa(v)beta(1a)-induced current amplitude was about two to four times higher than that of LoCavPlb. Experiments with point mutants and chimeras suggest that potential PKC and CK2 phosphorylation sites in the N-terminal region of LoCa(v)beta(1a) affect the current amplitude reciprocally, and may be responsible for regulating current amplitude. (C) 2003 Elsevier Science Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
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