期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
卷 135, 期 1, 页码 39-48出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S1532-0456(03)00047-4
关键词
microcystin-LR; goldfish; liver; aminotransferases; glutathione-S-transferase (GST); protein phosphatase; lactate dehydrogenase (LDH)
After intraperitoneal injection of microcystin-LR (MC-LR) (125 mug kg(-1) body wt.), the concentration of MC-LR in the liver of juvenile goldfish Carassius auratus (30 g body wt.) was assayed by a modified protein phosphatase inhibition method. A temporary accumulation occurred from 3 to 48 h post-injection, followed by a significant decrease between 48 and 96 h. Under our experimental conditions, contamination by MC-LR did not change ionic homeostasis, as attested by blood osmolality values and gill Na+ /K+ ATPase activity. Light microscopy observations revealed lesions and cellular necrosis progression, which was concomitant with an increase in enzyme activity of plasma aspartate aminotransferase (AspAT), alanine aminotransferase (AlaAT) and L-lactate dehydrogenase (LDH) and with a decrease of hepatic glutathione-S-transferase (GST) activity. Structural alterations and enzymatic activity modifications became significant within 24 h post-injection. Recovery of hepatocytes on day 21 after MC-LR injection was evident, together with a decrease in the MC-LR equivalent content of the liver. (C) 2003 Elsevier Science Inc. All rights reserved.
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