期刊
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
卷 95, 期 5, 页码 435-440出版社
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/S1389-1723(03)80041-5
关键词
acyl-CoA oxidase; acyl-CoA synthetase; free fatty acids; Saccharomyces cerevisiae; secretion
To elucidate the molecular mechanism governing fatty acid transport across the cell membrane, we first isolated a Saccharomyces cerevisiae mutant, B-1, that exhibits a reduced acyl-CoA oxidase activity and an increase in free fatty acid accumulation. Following mutagenesis of B-1, a mutant, YTS51, which secretes free fatty acids, was isolated. The concentration of free fatty acids in the YTS51 culture medium was about 17 times higher than that in B-1. The mutation that causes the fatty acid secretion phenotype occurred at a single allele, and this phenotype was suppressed by the introduction of a single copy of FAA1, a gene for acyl-CoA synthetase, to the mutant. Although the mutation expressing this phenotype was not within FAA1 in YTS51, the disruption of FAA1 in the wild-type strain resulted in fatty acid secretion even though the level of fatty acid secretion was less than that in YTS51. We consider that YTS51 is a suitable model to elucidate the molecular basis of the fatty acid transport process.
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