期刊
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY
卷 82, 期 1-3, 页码 121-135出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0079-6107(03)00010-5
关键词
stretch-activated ion channels; adaptation; pressure-clamp; atrium; rat
The effect, of mechanical stress on the heart's electrical activity has been termed mechanoelectric feedback. The response to stretch depends upon the magnitude and the waveform of the stimulus, and upon the timing relative to the cardiac cycle. Stretch-activated ion channels (SACs) have been regarded as the most likely candidates for serving as the primary transducers of mechanical stress. We explored the steady state and dynamic responses of single channels in adult rat atrial cells using the patch clamp with a pressure clamp. Surprisingly, we only observed K+-selective SACs, probably of the 2P domain family. The channels were weakly outward rectifying with flickery bursts. In cell attached mode, the mean conductance was 74+/-14 and 65+/-16 pS for +60 and -60mV, respectively (140 mM [K+](out), 2 mM [Mg2+](out) and 0 mM [Ca2+](out)). The latency of the response to pressure steps was 50-100 ms and the time to peak similar to400 ms. About half of the channels in cell-attached patches showed adaptation/inactivation where channel activity declined to a plateau of 20-30% of peak in similar to1 s. The time dependent behavior of these SACs is generally consistent with whole-cell currents observed in chick and rat ventricular cells, although the net current was outward rather than inward. (C) 2003 Elsevier Science Ltd. All rights reserved.
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