期刊
JOURNAL OF PROTEOME RESEARCH
卷 2, 期 3, 页码 313-319出版社
AMER CHEMICAL SOC
DOI: 10.1021/pr0300011
关键词
semiconductor microchip; protein microarray; in vitro translation; self-assembly; fluorescence; electrochemical detection
资金
- NHGRI NIH HHS [R43 HG 02461-01] Funding Source: Medline
Protein arrays will greatly accelerate research and development in medical and biological sciences. We have used cell-free protein biosynthesis and a parallel immobilization strategy for producing protein biochips. We demonstrate a model two-protein microarray using luciferase and green fluorescent protein, both expressed in a cell-free system and specifically immobilized on CombiMatrix semiconductor oligonucleotide microarrays. This demonstration provides evidence for the appropriate folding, activity, robust presentation, and efficient flexible detection of proteins on the microscale.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据