期刊
FERTILITY AND STERILITY
卷 79, 期 5, 页码 1187-1193出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S0015-0282(03)00071-2
关键词
intracytoplasmic sperm injection; FSH and LH receptors; messenger RNA; reverse transcription-polymerase chain reaction
Objective: To study the expression of the FSH and LH receptors in human oocytes and preimplantation embryos and their potential roles in early human development. Design: Clinical and molecular studies. Setting: University hospital IVF center. Patient(s): Female volunteers undergoing intracytoplasmic sperm injection at the IVF unit of the Athens University Hospital. All patients gave written informed consent. Intervention(s): Ovarian stimulation was performed with exogenous gonadotropin administration. Intracytoplasmic sperm injection was performed on mature oocytes. Main Outcome Measure(s): Unfertilized oocytes and zygotes and embryos at the 2-cell, 4-cell, morula, and blastocyst stage were selected for study. A polymerase chain reaction methodology was used to analyze human oocytes and embryos. Messenger RNA was reverse transcribed and amplified with FSH and LH receptor specific primers. Result(s): Transcripts for the FSH receptor were detected in oocytes and zygotes and embryos at the 2-cell, morula, and blastocyst stage, while no message was detected in embryos at the 4-cell stage. Transcripts for the LH receptor were observed in oocytes and zygotes and morula- and blastocyst-stage embryos, whereas no message was detected in embryos at the 2-cell and 4-cell stage. Conclusion(s): Messenger RNA for the FSH and LH receptors was observed in oocytes and preimplantation embryos at different stages, indicating a physiological role in the oocyte maturation process and early embryonic development in the human. (C) 2003 by American Society for Reproductive Medicine.
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