期刊
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
卷 51, 期 5, 页码 605-612出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/002215540305100506
关键词
fluorescence microscopy; energy-filtered transmission electron microscopy; electron spectroscopic imaging; nuclear structure; nuclear bodies; promyelocytic leukemia
类别
Correlative imaging of a specific cell with both the light microscope and the electron microscope has proved to be a difficult task, requiring enormous amounts of patience and technical skill. We describe a technique with a high rate of success, which can be used to identify a particular cell in the light microscope and then to embed and thin-section it for electron microscopy. The technique also includes a method to obtain many uninterrupted, thin serial sections for imaging by conventional or energy-filtered transmission electron microscopy, to obtain images for 3D analysis of detail at the suborganelle level.
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