期刊
PHYTOCHEMICAL ANALYSIS
卷 14, 期 3, 页码 145-149出版社
JOHN WILEY & SONS LTD
DOI: 10.1002/pca.695
关键词
fluorometry; flow assay; acetylcholinesterase inhibitor; fluorogenic substrate; 7-acetoxy-1-methyl quinolinium iodide; Amaryllidaceae
A fluorometric assay for acetylcholinesterase inhibitory activity was developed in a flow system using the fluorogenic substrate 7-acetoxy-1-methyl quinolinium iodide which is hydrolysed to the highly fluorescent 7-hydroxy-1-methyl quinolinium iodide. The detection limit of galanthamine is 0.5 muM, which is about 20 times more sensitive than in the colorimetric flow assay. In the presence of 30% methanol or of 5% acetonitrile, about 70% of the enzyme activity could still be detected. Various plant extracts have been screened using the described system including bulbs of Galanthus nivalis, Eucharis amazonica (E. x grandiflora), Crinum powelli and Nerine bowdenii (all members of the Amaryllidaceae), which showed strong AchE inhibitory activity. Copyright (C) 2003 John Wiley Sons, Ltd.
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