期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 67, 期 5, 页码 1140-1143出版社
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.67.1140
关键词
proanthocyanidin; procyanidin B1; absorption; grape seed extract; human
To confirm the absorption of proanthocyanidin (PA) into the human body, four healthy adults were administered 2.0 g of PA-rich grape seed extract (GSE). Blood were drawn before intake and 2 h after intake. Through the enzymatic treatment of sulfatase and beta-glucuronidase, blood samples were analyzed by HPLC coupled with mass-spectrometry (LC/MS). Procyanidin B1 [epicatechin-(4beta-->8)-catechin] was detected in human serum 2 h after intake. Its concentration was 10.6+/-2.5 nmol/l.
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