期刊
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE
卷 81, 期 5, 页码 517-522出版社
NATL RESEARCH COUNCIL CANADA
DOI: 10.1139/B03-032
关键词
Stevia rebaudiana Bertoni; Asteraceae; isoprenoids; 2-C-methyl-D-erythritol 4-phosphate pathway; 1-deoxy-D-xylulose-5-phosphate synthase; 1-deoxy-D-xylulose-5-phosphate reductoisomerase
The ent-kaurene skeleton of chloroplast diterpene glycosides, which are produced in large quantities in the leaves of Stevia rebaudiana Bertoni, is formed via the recently discovered 2-C-methyl-D-erythritol 4-phosphate pathway. The enzymes catalyzing the first two steps of this pathway, 1-deoxy-D-xylulose-5-phosphate synthase (DXS) and 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) were characterized. Using reverse transcriptase-PCR, the dxs and dxr cDNAs were cloned, which comprise ORFs of 2148 and 1422 nucleotides, respectively. The cDNA-derived amino acid sequences for DXS and DXR contain 716 and 474 residues, encoding polypeptides of about 76.6 and 51 kDa, respectively. DXS and DXR from Stevia both contain an N-terminal plastid targeting sequence and show high homology to other known plant DXS and DXR enzymes. Furthermore, we demonstrated through heterologous expression in Escherichia coli that the cloned cDNAs encode functional proteins.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据