期刊
GENE EXPRESSION PATTERNS
卷 3, 期 2, 页码 231-236出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S1567-133X(03)00036-X
关键词
rapid amplification of cDNA ends; ankyrin repeats; germ cell; in silico subtraction
资金
- NICHD NIH HHS [HD-07495] Funding Source: Medline
To discover germ cell-specific genes, we used in silico subtraction and identified testis expressed gene 14 (Tex14). Mouse Tex14 contains an open reading frame encoding a 1450-amino-acid protein, which shares 64% amino acid identity with the predicted human TEX14 protein. The predicted TEX14 amino acid sequence consists of three ankyrin repeats, a protein kinase domain, and a leucine zipper dimerization motif. Northern blot analysis and in situ hybridization show that Tex14 mRNA is expressed specifically in the testis, with highest levels observed in pachytene, diplotene, and meiotically dividing spermatocytes. Two 5' splice variants of mouse Tex14 were discovered by sequencing 5'-RACE polymerase chain reaction products. TEX14 is predicted to be localized to the nucleus, suggesting that it may play a key role in regulating gene expression or modulating nuclear events during mammalian spermatogenesis. (C) 2003 Published by Elsevier Science B.V.
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