期刊
JOURNAL OF CELL BIOLOGY
卷 161, 期 3, 页码 625-639出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200303020
关键词
NCAM; spectrin; PKC; neurons; outgrowth
类别
In hippocampal neurons and transfected CHO cells, neural cell adhesion molecule (NCAM) 120, NCAM140, and NCAM180 form Triton X-100-insoluble complexes with 01 spectrin. Heteromeric spectrin (alphalbetal) binds to the intracellular domain of NCAM180, and isolated spectrin subunits bind to both NCAM180 and NCAM140, as does the PI spectrin fragment encompassing second and third spectrin repeats (betal(2-3)). In NCAM120-transfected cells, betal spectrin is detectable predominantly in lipid rafts. Treatment of cells with methyl-beta-cyclodextrin disrupts the NCAM120-spectrin complex, implicating lipid rafts as a platform linking NCAM120 and spectrin. NCAM140/NCAM180-betal spectrin complexes do not depend on raft integrity and are located both in rafts and raft-free membrane domains. PKCbeta(2) forms detergent-insoluble complexes with NCAM140/NCAM180 and spectrin. Activation of NCAM enhances the formation of NCAM140/NCAM180-spectrin-PKCbeta(2) complexes and results in their redistribution to lipid rafts. The complex is disrupted by the expression of dominant-negative betal(2-3), which impairs binding of spectrin to NCAM, implicating spectrin as the bridge between PKCbeta(2) and NCAM140 or NCAM180. Redistribution of PKCbeta(2) to NCAM-spectrin complexes is also blocked by a specific fibroblast growth factor receptor inhibitor. Furthermore, transfection with betal(2-3) inhibits NCAM-induced neurite outgrowth, showing that formation of the NCAM-spectrin-PKCbeta(2) complex is necessary for NCAM-mediated neurite outgrowth.
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