期刊
DNA REPAIR
卷 2, 期 5, 页码 571-580出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S1568-7864(03)00023-5
关键词
mismatch repair; FEN-1; Exo1; mutation avoidance; DNA repeats
A frameshift reversion assay has been established for Schizosaccharomyces pombe, which allows detection of deletions and insertions of nucleotides in a non-repetitive DNA sequence. Compared to wild type, frameshift mutation rates were increased in the mismatch repair (MMR) mutants msh2, msh6, mlh1, and pms1, but not in a swi4 strain (defective in the Msh3 homologue). Rates were also elevated in the DNA nuclease-deficient strains rad2 (defective in the FEN-1 homologue) and exol. In MutSalpha-deficient strains, msh2 and msh6, most of the reversions were 1 bp deletions. In contrast, mlh1 and pms1 mutants, defective in MutLalpha, accumulated significantly more 2 bp insertions, preferentially of the type CG to (CG)(2). Such duplications were less frequent in double mutants additionally defective in msh2, msh6, rad2, or exol. Thus, accumulation of (CG)(2) in MutLalpha-deficient strains depends on the presence of MutSalpha, Rad2 and Exol. (C) 2003 Elsevier Science B.V All rights reserved.
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