期刊
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
卷 1627, 期 1, 页码 39-46出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0167-4781(03)00072-1
关键词
human 80S ribosome; photoaffinity cross-linking; mRNA analog; mRNA binding center; ribosomal RNA
Positioning of each nucleotide of the E site and the P site bound codons with respect to the 18S rRNA on the human ribosome was studied by cross-linking with mRNA analogs, derivatives of the hexaribonucleotide UUUGUU (comprising Phe and Val codons) that carried a perfluorophenylazide group on the second or the third uracil, and a derivative of the dodecaribonucleotide UUAGUAUUUAUU with a similar group on the guanine residue. The location of the modified nucleotides at any mRNA position from - 3 to + 3 (position + I corresponds to the 5' nucleotide of the P site bound codon) was adjusted by the cognate tRNAs. A modified uridine at positions from - I to + 3 cross-linked to nucleotide G1207 of the 18S rRNA, and to nucleotide G961 when it was in position - 2. A modified guanosine crosslinked to nucleotide G1207 if it was in position - 3 of the mRNA. These data indicate that nucleotide G961 of the 18S rRNA is close only to mRNA positions - 3 and - 2, while G1207 is in the vicinity of positions from - 3 to + 3. The latter suggests that there is a sharp turn between the P and E site bound codons that brings nucleotide G1207 of the 18S rRNA close to each nucleotide of these codons. This correlates well with X-ray crystallographic data on bacterial ribosomes, indicating existence of a sharp turn between the P site and E site bound codons near a conserved nucleotide G926 of the 16S rRNA (corresponding to G 1207 in 18S rRNA) close to helix 23b containing the conserved nucleotide 693 of the 16S rRNA (corresponding exactly to G961 of the 18S rRNA). (C) 2003 Elsevier Science BY, All rights reserved.
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