期刊
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY
卷 36, 期 1-2, 页码 27-32出版社
WILEY
DOI: 10.1016/S0928-8244(03)00038-5
关键词
Touch-Down-PCR; Mycobacterium leprae; drug resistance; mutation
The lack of methods to identify Mycobacterium leprae with the resistance against multi-drugs quickly and specifically has hindered effective chemotherapy against M. leprae infection. To screen M. leprae with resistance against multi-drugs, the Touch-Down (TD)-PCR has been used in this study. Sequences of the folP, rpoA, B, and gyrA, B genes were analyzed for isolates of M. leprae from leprosy patients in Korea. We amplified designated region of several genes in M. leprae involved in drug resistance and could obtain the PCR products of each gene. The mutations in the particular region of folP, rpoB, and gyrB gene were certified by TD-PCR single-stranded conformational polymorphism and DNA sequencing, respectively. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据