期刊
JOURNAL OF SURGICAL RESEARCH
卷 111, 期 2, 页码 261-266出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S0022-4804(03)00087-8
关键词
interleukin 6; vascular smooth muscle cell; migration; actin polymerization; focal adhesion kinase; paxillin
类别
Background. Interleukin 6 (IL-6) is elevated in the arterial wall in atherosclerosis and restenosis after angioplasty. An important contributor to these pathologies is migration of vascular smooth muscle cells (VSMC), which is often associated with cytoskeletal. reorganization initiated by growth factors and chemokines. We recently reported that IL-6 stimulated migration of VSMC. Here, we examined the cytoskeleton of VSMC and cytoskeletal. associated proteins to determine potential mechanisms associated with IL-6 induced migration. Materials and methods. Studies were performed in VSMC cultured from rat aortas. Results. IL-6 significantly stimulated VSMC migration. IL-6 induced actin polymerization, and tyrosine phosphorylation of focal adhesion-associated cytoskeletal proteins including focal adhesion kinase (FAK) and paxillin. Cytochalasin D, an inhibitor of actin polymerization, blocked phosphorylation of FAK and paxillin as well as cell motility induced by the cytokine. Conclusions. Collectively, these data demonstrate for the first time that IL-6 stimulates VSAIC motility which correlated with induction of actin cytoskeletal reorganization and tyrosine phosphorylation. of FAK and paxillin. (C) 2003 Elsevier Inc. All rights reserved.
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