期刊
OPTICS EXPRESS
卷 11, 期 10, 页码 1123-1130出版社
Optica Publishing Group
DOI: 10.1364/OE.11.001123
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A dedicated two-photon microscope incorporating adaptive-optic correction of specimen-induced aberrations is presented. Wavefront alteration of the scanning laser beam was achieved by use of a micromachined deformable mirror. Post scan head implementation produces a compact module compatible with the Bio-Rad MRC-600 scan head. Automatic aberration correction using feedback from the multiphoton fluorescence intensity allowed the adaptive optic to extend the imaging depth attainable in both artificial and biological refractive-index mismatched samples. With a 1.3-NA, x40, Nikon oil immersion objective, the imaging depth in water was extended from approximately 3.4 to 46.2 mum with a resolution defined by a FWHM axial point-spread function of 1.25 mum.. 2003 Optical Society of America.
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