期刊
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
卷 34, 期 6, 页码 1162-1170出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/ATVBAHA.114.302528
关键词
atherosclerosis; lipoproteins; lymphatic vessels
资金
- Academy of Finland
- Academy of Finland Collaborative Research Consortium on Genome-Scale Cancer Biology [262976]
- Center of Excellence in Cardiovascular Diseases and Type 2 Diabetes
- European Research Council (FUTUREGENES) [250050]
- European Research Council (TX-FACTORS) [268804]
- Leducq Transatlantic Network of Excellence (Lymph Vessels in Obesity and Cardiovascular Disease)
- Finnish Foundation for Cardiovascular disease
- Kuopio University Hospital (EVO grant)
- National Institutes of Health/National Center for Research Resources Colorado CTSI [UL1 RR025780]
- [R21 HL108177]
- European Research Council (ERC) [250050, 268804] Funding Source: European Research Council (ERC)
- Academy of Finland (AKA) [262976, 262976] Funding Source: Academy of Finland (AKA)
Objective Lymphatic vessels collect extravasated fluid and proteins from tissues to blood circulation as well as play an essential role in lipid metabolism by taking up intestinal chylomicrons. Previous studies have shown that impairment of lymphatic vessel function causes lymphedema and fat accumulation, but clear connections between arterial pathologies and lymphatic vessels have not been described. Approach and Results Two transgenic mouse strains with lymphatic insufficiency (soluble vascular endothelial growth factor 3 [sVEGFR3] and Chy) were crossed with atherosclerotic mice deficient of low-density lipoprotein receptor and apolipoprotein B48 (LDLR-/-/ApoB(100/100)) to study the effects of insufficient lymphatic vessel transport on lipoprotein metabolism and atherosclerosis. Both sVEGFR3xLDLR(-/-)/ApoB(100/100) mice and ChyxLDLR(-/-)/ApoB(100/100) mice had higher plasma cholesterol levels compared with LDLR-/-/ApoB(100/100) control mice during both normal chow diet (16.3 and 13.7 versus 8.2 mmol/L, respectively) and Western-type high-fat diet (eg, after 2 weeks of fat diet, 45.9 and 42.6 versus 30.2 mmol/L, respectively). Cholesterol and triglyceride levels in very-low-density lipoprotein and low-density lipoprotein fractions were increased. Atherosclerotic lesions in young and intermediate cohorts of sVEGFR3xLDLR(-/-)/ApoB(100/100) mice progressed faster than in control mice (eg, intermediate cohort mice at 6 weeks, 18.3% versus 7.7% of the whole aorta, respectively). In addition, lesions in sVEGFR3xLDLR(-/-)/ApoB(100/100) mice and ChyxLDLR(-/-)/ApoB(100/100) mice had much less lymphatic vessels than lesions in control mice (0.33% and 1.07% versus 7.45% of podoplanin-positive vessels, respectively). Conclusions We show a novel finding linking impaired lymphatic vessels to lipoprotein metabolism, increased plasma cholesterol levels, and enhanced atherogenesis.
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