Physiological basis for antagonism of clethodim by imazapic on goosegrass (Eleusine indica (L.) Gaertn.)

Greenhouse and laboratory experiments were conducted to determine the effect of imazapic on the herbicidal activity of clethodim on goosegrass. Imazapic did not affect absorption of [C-14]clethodim by goosegrass. Averaged across the two treatments of clethodim alone and clethodim plus imazapic, absorption was 36 and 89% of applied [C-14]Cle- thodim at 0.5 and 96 h, respectively. The majority of [C-14]clethodim (19% of applied) was absorbed by 24h. Translocation of C-14 was not affected by imazapic, and 3.6% of applied C-14 had translocated into the portion of the shoot below the treated leaf at 96 h after treatment. Metabolism of clethodim was not affected by the presence of imazapic. Three major metabolites of clethodim, were detected in treated tissue at all harvest intervals. The majority (58%) of [C-14]clethodim was converted to a relative polar metabolite form 96h after treatment, whether clethodim was applied alone or in the presence of imazapic. One day after treatment, the photosynthetic rate in plants treated with imazapic decreased below the rate in the non-treated check, and was less for 8 days, the duration of the study. These data suggest that the antagonism of clethodim by imazapic may be caused by imazapic reducing the photosynthetic rate of goosegrass and therefore the sensitivity of ACCase to clethodim. (C) 2003 Elsevier Science (USA). All rights reserved.