期刊
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
卷 32, 期 6, 页码 1505-+出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/ATVBAHA.112.249854
关键词
atherosclerosis; carotid plaque; inflammation; lipoprotein-associated phospholipase A2; lysophosphatidylcholine
资金
- GlaxoSmithKline
- Swedish Research Council [K2011-65X-08311-24-6, K2011-65X-21753-01-6]
- Marianne and Marcus Wallenberg Foundation
- Swedish Heart and Lung Foundation [20080434, 20090419, 20090490]
- Swedish Medical Society
- Swedish Medical Society, VINNOVA [2009-00164]
- Swedish Foundation for Strategic Research [RBa08-0075]
- Soderbergs's, Zoega's, Lundgren's
- German Federal Ministry of Education and Research (BMBF)
- Formas [2009-00164] Funding Source: Formas
- Vinnova [2009-00164] Funding Source: Vinnova
- Swedish Foundation for Strategic Research (SSF) [RBa08-0075] Funding Source: Swedish Foundation for Strategic Research (SSF)
Objective-To determine whether the level of lysophosphatidylcholine (lysoPC) generated by lipoprotein-associated phospholipase A2 (Lp-PLA2) is associated with severity of inflammation in human atherosclerotic plaques. Elevated plasma Lp-PLA2 is associated with increased cardiovascular risk. Lp-PLA2 inhibition reduces atherosclerosis. Lp-PLA2 hydrolyzes low-density lipoprotein-oxidized phospholipids generating lysoPCs. According to in vitro studies, lysoPCs are proinflammatory but the association between their generation and plaque inflammation remains unknown. Methods and Results-Inflammatory activity in carotid plaques (162 patients) was determined immunohistochemically and by analyzing cytokines in homogenates (multiplex immunoassay). LysoPCs were quantified using mass spectrometry and Lp-PLA2 and the lysoPC metabolite lysophosphatidic acid (LPA) by ELISA. There was a strong correlation among lysoPC 16: 0, 18: 0, 18: 1, LPA, and Lp-PLA2 in plaques. LysoPC 16: 0, 18: 0, 18: 1, LPA, and Lp-PLA2 correlated with interleukin-1 beta, interleukin-6, monocyte chemoattractant protein-1, macrophage inflammatory protein-1 beta, regulated on activation normal T-cell expressed and secreted, and tumor necrosis factor-alpha in plaques. High lysoPC and Lp-PLA2 correlated with increased plaque macrophages and lipids and with low content of smooth muscle cells, whereas LPA only correlated with plaque macrophages. Lp-PLA2, lysoPC 16: 0, 18: 0, and 18: 1, but not LPA were higher in symptomatic than in asymptomatic plaques. Conclusion-The associations among Lp-PLA2, lysoPCs, LPA, and proinflammatory cytokines in human plaques suggest that lysoPCs play a key role in plaque inflammation and vulnerability. Our findings support Lp-PLA2 inhibition as a possible strategy for the prevention of cardiovascular disease. (Arterioscler Thromb Vasc Biol. 2012;32:1505-1512.)
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