期刊
CELLULAR AND MOLECULAR LIFE SCIENCES
卷 60, 期 6, 页码 1099-1106出版社
SPRINGER BASEL AG
DOI: 10.1007/s00018-003-2253-2
关键词
cell cycle; synchronization; double-thymidine block; G1 phase arrest; nocodazole; mitotic arrest
An analysis of different classes of forced or batch synchronization methods reveals why these methods, in theory, do not produce synchronized cultures. Cells may be aligned for a particular property after specific treatments, but these aligned cells do not correspond to any particular cell age during the normal cell cycle. The experimental methods analyzed are those that arrest cells with a G1 phase amount of DNA, those that inhibit DNA synthesis, and those that arrest cells at mitosis. Release of arrested cells from inhibition does not produce cells reflecting cells during the normal division cycle. Thus, cells produced by batch or forcing methods are not experimental models for analysis of the normal cell cycle.
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