期刊
SOIL BIOLOGY & BIOCHEMISTRY
卷 35, 期 6, 页码 775-782出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0038-0717(03)00105-6
关键词
root exudates; soil bacterial community; soil respiration; denaturing gradient gel electrophoresis
类别
Simulating the evolution of both C-14 and C-12-CO2 in the rhizoplane was monitored during the diffusion of C-14-labelled glucose, oxalic acid, or glutamic acid into soil from a filter placed on the surface of a sandy loam. After 3 and 7 d, soil was sampled from four layers (0-2, 2-4, 4-6, and 6-14 mm) to determine residual C-14 in each layer. The mineralisation pattern of oxalic acid was characterised by a lag phase probably due to the presence, in the early stages of exposure, of a few microorganisms able to mineralise this substrate. Glucose and glutamic acid showed a positive priming effect with a CO2 flush from native organic matter. Oxalic and glutamic acids changed the denaturing gradient gel electrophoresis profiles of soil bacterial communities with the appearance of a few extra-bands in the 0-2 mm soil layer. The addition of the substrates onto the soil surface formed a gradient due to their diffusion in soil. That of oxalic acid was specific probably because almost all of this compound reacted with CaCO3, and was localised in the 0-2 mm soil layer. (C) 2002 Elsevier Science Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据