期刊
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
卷 29, 期 8, 页码 1213-1219出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/ATVBAHA.109.189407
关键词
apoptosis; inflammation; Mcl-1; interferon-gamma; LMP7; PSMB8; Fas; immunoproteasome
资金
- National Institutes on Aging [AG12712]
- The Cathearine Birch McCormick Genomics Center
- St. Laurent Institute
Objective-Increasing evidence suggests that chronic inflammation contributes to atherogenesis, and that acute inflammatory events cause plaque rupture, thrombosis, and myocardial infarction. The present studies examined how inflammatory factors, such as interferon-gamma (IFN gamma), cause increased sensitivity to apoptosis in vascular lesion cells. Methods and Results-Cells from the fibrous cap of human atherosclerotic lesions were sensitized by interferon-gamma (IFN gamma) to Fas-induced apoptosis, in a Bcl-X-L reversible manner. Microarray profiling identified 72 INF gamma-induced transcripts with potential relevance to apoptosis. Half could be excluded because they were induced by IRF-1 overexpression, which did not sensitize to apoptosis. IFN gamma treatment strongly reduced Mcl-1, phospho-Bcl-2 (ser70), and phospho-Bcl-X-L (ser62) protein levels. Candidate transcripts were modulated by siRNA, overexpression, or inhibitors to assess the effect on IFN gamma-induced Fas sensitivity. Surprisingly, siRNA knockdown of PSMB8 (LMP7), an immunoproteasome component, reversed IFN gamma induced sensitivity to Fas ligation and prevented Fas/IFN gamma-induced degradation of Mcl-1, but did not protect p-Bcl-2 or p-Bcl-X-L. Proteasome inhibition markedly increased Mcl-1, p-Bcl-2, and p-Bcl-X-L levels after IFN gamma treatment. Conclusions-Although critical for antigen presentation, the immunoproteasome appears to be a key link between inflammatory factors and the control of vascular cell apoptosis and may thus be an important factor in plaque rupture and myocardial infarction. (Arterioscler Thromb Vasc Biol. 2009; 29: 1213-1219.)
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