期刊
JOURNAL OF CELL SCIENCE
卷 116, 期 11, 页码 2303-2310出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.00446
关键词
Golgi; phospholipase A(2); cyclooxygenase; arachidonic acid; calcium; Ca2+
类别
Cytosolic phospholipase A(2)-alpha (cPLA(2)-alpha) is an 85 kDa, Ca2+-sensitive enzyme involved in receptor-mediated prostaglandin synthesis. In airway epithelial cells, the release of prostaglandins is crucial in regulating the inflammatory response. Although prostaglandin release has been studied in various epithelial cell models, the subcellular location of cPLA(2)-alpha in these cells is unknown. Using high-resolution confocal microscopy of the human A549 lung epithelial cell line, we show that cPLA(2)-alpha relocates from the cytosol and nuclei to a juxtanuclear region following stimulation with the Ca2+ ionophore A23187. Double staining with rhodamine-conjugated wheat germ agglutinin confirmed this region to be the Golgi apparatus. Markers specific for Golgi subcompartments revealed that cPLA(2)-alpha is predominantly located at the traps-Golgi stack and the traps-Golgi network following elevation of cytosolic Ca2+. Furthermore, treatment of cells with the Golgi-disrupting agent brefeldin A caused a redistribution of cPLA(2)-alpha, confirming that cPLA(2)-alpha associates with Golgi-derived membranes. Finally, a specific co-localization of cPLA(2)-alpha with cyclooxygenase-1 but not cyclooxygenase-2 was evident at the Golgi apparatus. These results, combined with recent data on the role of PLA(2) activity in maintaining Golgi structure and function, suggest that Golgi localization of cPLA(2)-alpha may be involved in membrane trafficking in epithelial cells.
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