期刊
ELECTROPHORESIS
卷 24, 期 12-13, 页码 2106-2110出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elps.200305418
关键词
chiral separation; cyclodextrin; methadone
A stereoselective method was developed for the simultaneous determination of methadone and its two major metabolites, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) and 2-ethyl-5-methyl-3,3-diphenyl-1-pyrroline (EMDP) by capillary electrophoresis. Five beta-cyclodextrin (betaCD) background electrolyte (BGE) additives were evaluated for resolution efficiency. The conditions for baseline resolution of each of the three enantiomer pairs was determined to be 1 mm heptakis-(2,6-di-O-methyl)beta-cyclodextrin(DM PCD) in 100 mm phosphate at pH 2.6. This method represents the first successful method for the resolution of the six enantionners associated with the metabolism of methadone. The utilisation of doubly coated capillaries in conjunction with betaCD derivatives for a faster separation of the methadone-related enantionners is also reported. The coated capillaries were prepared using a polycation of poly(diallyldimethylammonium chloride) (PDDAC) and a polyanion of dextran sulfate. Baseline resolution of the methadone enantionners was achieved with a BGE of 8 mm (2-hydroxy)propyl-p-cyclodextrin (HPbetaCD) in 100 mM phosphate at pH 2.6. The migration times for the stereoselective methadone separation were approximately 4 min, which represented a reduction by a factor of approximately three, compared to that attained using analogous conditions with the uncoated capillary.
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