期刊
CHINESE SCIENCE BULLETIN
卷 48, 期 12, 页码 1247-1250出版社
SCIENCE PRESS
DOI: 10.1360/02wc0520
关键词
JDV; cellular factor AP-4; trans-activator (Tat); LTR; site-directed mutagenesis
Previous studies with deletion and sequence analysis of JDV LTR showed that there is a putative AP-4 responsive element in LTR. By antisense transient assay and gel shifting assay, we for the first time demonstrated that AP-4 modulated JDV gene expression by binding DNA directly to bovine cells. The results, derived from site-directed mutagenesis experiments, suggest that the six base pairs of AP-4 binding site (CAGCTG) have different effects on JDV gene expression. When the first two base pairs changed to GC, JDV gene expression is severely decreased.
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