期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 376, 期 4, 页码 440-443出版社
SPRINGER-VERLAG BERLIN
DOI: 10.1007/s00216-003-1756-x
关键词
HPLC; pharmaceuticals; degradation product; methylparaben; propylparaben; triamcinolone acetonide
A novel reversed-phase high-performance liquid chromatographic (RP-HPLC) method was developed and validated for the determination of active component triamcinolone acetonide, its degradation product triamcinolone (occurring in formulation after long-term stability tests) and two preservatives presented in the cream-methylparaben and propylparaben, using hydrocortisone as an internal standard. The chromatographic separation was performed on a Supelco Discovery C18 column; the mobile phase for separation of all compounds consists of a mixture of acetonitrile and water (40:60 v/v). The analysis time was less than 9 min, at a flow rate of 0.6 mL min(-1) and detection at 240 nm. The method was found to be applicable for routine analysis (stability tests, homogeneity) in the pharmaceutical product topical cream Triamcinolon cream 0.1%.
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