期刊
BIOTECHNOLOGY LETTERS
卷 25, 期 11, 页码 895-899出版社
SPRINGER
DOI: 10.1023/A:1024038609045
关键词
microinjection; optoporation; photodynamic treatment; transfection
A pulsed (17 nanoseconds) Nd: YAG laser (1064 nm) was used to inject impermeable dyes (propidium iodide andiodide and merocyanine 540) and a plasmid (pEGFP-N1) encoding green fluorescent protein (GFP) into human breast adenocarcinoma cells (MCF-7). The cell membrane integrity and viability were fully preserved in this laser-assisted transfer.
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